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Promega 5× green gotaq reaction buffer
5× Green Gotaq Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+green+gotaq+reaction+buffer/pcr+buffer/pm40016670-219-20-24
Average 90 stars, based on 1 article reviews
5× green gotaq reaction buffer - by Bioz Stars, 2026-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.
Article Snippet: .. Each PCR reaction was composed of 32.75 μL Milli-Q water, 10 μL 5× Green GoTaq Reaction Buffer (Promega), 1 μL dNTPs mix (each deoxynucleotide at 10 mM), 2 μL of each primer (10 μM), 0.25 μL GoTaq DNA Polymerase (Promega), and 2 μL of extracted DNA. .. When faint or no bands were observed on agarose gel electrophoresis following PCR, DNA extraction was repeated with DNeasy Blood&Tissue Kit (Qiagen) following the manufacturer’s recommendations.

Article Title: Evolution and biogeography of <i>Anablepsoides</i> killifishes shaped by Neotropical geological events (Cyprinodontiformes, Aplocheilidae)
Article Snippet: The Neotropical freshwater ichthyofauna is composed of more than 6,000 described species, most of them inhabiting river basins associated with the Amazon river (Fricke et al., 2022; Jézéquel et al., 2020; Reis et al., 2003, 2016).. This diversity is probably even greater, and estimations indicate that there may be more than 9000 freshwater fish species in this region (Albert et al., 2020; Brosse et al., 2013; Reis et al., 2016).. Despite this vast diversity, there is still little information about the biogeographical history of most of those lineages.

Article Title: Demonstration of Insect Vector-Mediated Transfer of a Betasatellite between Two Helper Viruses
Article Snippet: .. Each PCR was performed in a final volume of 25 μL containing 5 μL of 5× Green GoTaq Reaction Buffer (Promega, Madison, WI, USA), 1 μL of 5 mM of each dNTP, primer mix as mentioned in , 1.25 units of GoTaq DNA polymerase (Promega, USA), and 1 μL of total DNA extract. ..

Article Title: Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion.
Article Snippet: Ear and tail samples, collected from adult and postnatal mice, were lysed in DirectPCR Lysis Reagent (Mouse Tail; Viagen, 102-T) overnight at 55°C. .. The following day, samples were boiled for 10 min. Gene fragments were amplified by polymerase chain reaction (PCR) with 5× Green GoTaq Reaction Buffer (Promega, M791A), dNTP mix (Promega, U1515), and GoTaq G2 DNA polymerase (Promega, M7848). .. PCR products were run on 1.5% agarose gels with 1X GelRed Nucleic Acid Stain (MilliporeSigma, SCT123) and visualized via iBright1500 imager (Invitrogen).

Article Title: In ovo administration of a phage cocktail partially prevents colibacillosis in chicks.
Article Snippet: .. The PCR reaction was performed as follows: each mix of 25 mL final volume contained the 13.3 mL of resuspended-colony in water, 5 mL of 5£ Green GoTaq reaction buffer (Promega, Madison, WI), 1.5 mL of MgCl2 (25 mM) (Promega), 0.5 mL of dNTP (10 mM) (Promega), 1.25 mL of each primer (10 mM) (Eurogentec, Liege, Belgium), and 0.2 mL of GoTaq DNA Polymerase (Promega). .. The thermocycling conditions were: 5 min at 94°C, 30 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C, and a final step of 5 min at 72°C (ProFlex, Applied Biosystems, Waltham, MA).

Article Title: In ovo administration of a phage cocktail partially prevents colibacillosis in chicks
Article Snippet: .. The PCR reaction was performed as follows: each mix of 25 μL final volume contained the 13.3 μL of resuspended-colony in water, 5 μL of 5× Green GoTaq reaction buffer (Promega, Madison, WI), 1.5 μL of MgCl 2 (25 mM) (Promega), 0.5 μL of dNTP (10 μM) (Promega), 1.25 μL of each primer (10 μM) (Eurogentec, Liege, Belgium), and 0.2 μL of GoTaq DNA Polymerase (Promega). .. The thermocycling conditions were: 5 min at 94°C, 30 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C, and a final step of 5 min at 72°C (ProFlex, Applied Biosystems, Waltham, MA).

Amplification:

Article Title: Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion.
Article Snippet: Ear and tail samples, collected from adult and postnatal mice, were lysed in DirectPCR Lysis Reagent (Mouse Tail; Viagen, 102-T) overnight at 55°C. .. The following day, samples were boiled for 10 min. Gene fragments were amplified by polymerase chain reaction (PCR) with 5× Green GoTaq Reaction Buffer (Promega, M791A), dNTP mix (Promega, U1515), and GoTaq G2 DNA polymerase (Promega, M7848). .. PCR products were run on 1.5% agarose gels with 1X GelRed Nucleic Acid Stain (MilliporeSigma, SCT123) and visualized via iBright1500 imager (Invitrogen).



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